Review



dna preparation streptococcus pyogenes cas9  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc dna preparation streptococcus pyogenes cas9
    (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, <t>Cas9-BirA*</t> programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.
    Dna Preparation Streptococcus Pyogenes Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+preparation+streptococcus+pyogenes+cas9/pmc07398558-615-5-11?v=Addgene+inc
    Average 93 stars, based on 37 article reviews
    dna preparation streptococcus pyogenes cas9 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells"

    Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2020.06.014

    (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, Cas9-BirA* programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.
    Figure Legend Snippet: (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, Cas9-BirA* programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.

    Techniques Used: Mass Spectrometry, Incubation, Plasmid Preparation, Isolation

    (A) Time course of Cas9 RNPs programmed against a linear DNA substrate in a 1:2 molar ratio in buffer, HSS with ATP, or HSS with CIP and ATPγS.
    Figure Legend Snippet: (A) Time course of Cas9 RNPs programmed against a linear DNA substrate in a 1:2 molar ratio in buffer, HSS with ATP, or HSS with CIP and ATPγS.

    Techniques Used:

    (A) HDR rates from amplicon-NGS sequencing of VEGFA 0, 1, 2, 3, 6, 9, 12, and 24 hours after electroporation of Cas9 RNPs in the presence of an HDR donor (N = 3 biological replicates). Data are represented as mean ± standard deviation. See also Figure S3E.
    Figure Legend Snippet: (A) HDR rates from amplicon-NGS sequencing of VEGFA 0, 1, 2, 3, 6, 9, 12, and 24 hours after electroporation of Cas9 RNPs in the presence of an HDR donor (N = 3 biological replicates). Data are represented as mean ± standard deviation. See also Figure S3E.

    Techniques Used: Amplification, Sequencing, Electroporation, Standard Deviation

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Purification, Recombinant, Amplification, Sequencing, Plasmid Preparation, Software



    Similar Products

    dna preparation streptococcus pyogenes cas9  (Addgene inc)
    93
    Addgene inc dna preparation streptococcus pyogenes cas9
    (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, <t>Cas9-BirA*</t> programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.
    Dna Preparation Streptococcus Pyogenes Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+preparation+streptococcus+pyogenes+cas9/pmc07398558-615-5-11?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    dna preparation streptococcus pyogenes cas9 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, Cas9-BirA* programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.

    Journal: Molecular cell

    Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells

    doi: 10.1016/j.molcel.2020.06.014

    Figure Lengend Snippet: (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, Cas9-BirA* programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.

    Article Snippet: Cas9, FACT, RNA, and Donor DNA Preparation Streptococcus pyogenes Cas9 (pMJ915, Addgene #69090) with two nuclear localization signal sequences and an HA tag at the C-terminus ( Lin et al., 2014 ) was expressed in Rosetta2 DE3 (QB3-Berkeley Macrolab) cells.

    Techniques: Mass Spectrometry, Incubation, Plasmid Preparation, Isolation

    (A) Time course of Cas9 RNPs programmed against a linear DNA substrate in a 1:2 molar ratio in buffer, HSS with ATP, or HSS with CIP and ATPγS.

    Journal: Molecular cell

    Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells

    doi: 10.1016/j.molcel.2020.06.014

    Figure Lengend Snippet: (A) Time course of Cas9 RNPs programmed against a linear DNA substrate in a 1:2 molar ratio in buffer, HSS with ATP, or HSS with CIP and ATPγS.

    Article Snippet: Cas9, FACT, RNA, and Donor DNA Preparation Streptococcus pyogenes Cas9 (pMJ915, Addgene #69090) with two nuclear localization signal sequences and an HA tag at the C-terminus ( Lin et al., 2014 ) was expressed in Rosetta2 DE3 (QB3-Berkeley Macrolab) cells.

    Techniques:

    (A) HDR rates from amplicon-NGS sequencing of VEGFA 0, 1, 2, 3, 6, 9, 12, and 24 hours after electroporation of Cas9 RNPs in the presence of an HDR donor (N = 3 biological replicates). Data are represented as mean ± standard deviation. See also Figure S3E.

    Journal: Molecular cell

    Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells

    doi: 10.1016/j.molcel.2020.06.014

    Figure Lengend Snippet: (A) HDR rates from amplicon-NGS sequencing of VEGFA 0, 1, 2, 3, 6, 9, 12, and 24 hours after electroporation of Cas9 RNPs in the presence of an HDR donor (N = 3 biological replicates). Data are represented as mean ± standard deviation. See also Figure S3E.

    Article Snippet: Cas9, FACT, RNA, and Donor DNA Preparation Streptococcus pyogenes Cas9 (pMJ915, Addgene #69090) with two nuclear localization signal sequences and an HA tag at the C-terminus ( Lin et al., 2014 ) was expressed in Rosetta2 DE3 (QB3-Berkeley Macrolab) cells.

    Techniques: Amplification, Sequencing, Electroporation, Standard Deviation

    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells

    doi: 10.1016/j.molcel.2020.06.014

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cas9, FACT, RNA, and Donor DNA Preparation Streptococcus pyogenes Cas9 (pMJ915, Addgene #69090) with two nuclear localization signal sequences and an HA tag at the C-terminus ( Lin et al., 2014 ) was expressed in Rosetta2 DE3 (QB3-Berkeley Macrolab) cells.

    Techniques: Purification, Recombinant, Amplification, Sequencing, Plasmid Preparation, Software